FastOcean ADP Profiling System for In Situ Estimation of Gross Primary Productivity (GPP)
FastOcean APD is a multi-wavelength Fast Repetition Rate fluorometer system for in situ estimation of gross primary producitivy (GPP). It is the tool of choice for monitoring variable chlorophyll fluorescence in photosynthetic organisms.
This autonomous profiling system comprises two FastOcean multi-wavelength Fast Repetition Rate fluorometers (ambient plus dark), pumped dark chamber, programmable battery pack and a PAR sensor for autonomous, in situ estimation of gross primary production. This system is smaller, lighter and more power efficient than earlier FRRf profiling systems.
This high performance system is suitable for use in a wide range of applications from the assessment of primary production within the world's oceans to environmental monitoring within reservoirs and rivers.
- GPP at Last?
GPP at Last?
Although fast repetition rate fluorometry (FRRf) has long been used for in situ estimation of electron flux through photosystem II (JPSII) and PSII photochemical efficiency (fPSII) on wide spatial and temporal scales, it has not been possible to convert these high frequency data to estimates of GPP, due to the difficulty in measuring photosystem II reaction centre concentration ([RCII]) or the absorption of light by the PSII light harvesting system, which are required for the processing of JPSII or fPSII data, respectively.
In a recent publication, *Oxborough et al. (2012) describe a new approach to the analysis of FRRf data, which includes methods for estimation of [RCII] and aLHII (absorption coefficient for PSII light harvesting) from FRRf data alone. The incorporation of this new approach to the analysis of FRRf data within multi-wavelength FastOcean sensors and FastPro8 software makes it possible to generate real time estimates of GPP, by combining measurements made under ambient light and after dark adaptation of two seconds. The FastOcean-based APD system uses separate sensors to make the required ambient and dark measurements in situ. As an alternative approach, PE curve data for estimation of GPP can be generated using a single FastOcean sensor, coupled with a FastAct laboratory system (see separate 'FastOcean plus FastAct' handout).
*Oxborough, K. Moore, C.M., Suggett, D.J., Lawson, T., Chan, H.G. and Geider, R.G. 2012 Direct estimation of functional PSII reaction centre concentration and PSII electron flux on a volume basis: a new approach to the analysis of Fast Repetition Rate fluorometry (FRRf) data. Limnol. Oceanogr.: Methods 10:142-154.
- Applications & Features
- Autonomous and real time profiling systems for in situ estimation of gross primary productivity (GPP)
- Sea-truthing of satellite data: Using new algorithms for the estimation of GPP and light absorption
- Fully automated synchronisation between ambient and dark sensors
- Three excitation wavelengths centred at 450, 530 and 624 nm
- Multiple excitation wavelength combinations can be run in a continuous cycle
- FastPro8 software provides automated data processing, presentation, archiving and export
- Automatic recalculation of all relevant parameters after the subtraction of sample blanks and other user-modifiable parameters
- Both of the FastOcean sensors within the APD system can be used for lab work, with or without FastAct systems
- Components & Specifications
Naming of parts: System Components: Part No Real time system Battery system 1 x FastOcean PTX (2220-140-PL) √ √ 1 x FastOcean to PAR sensor cable (2220-168-PL) √ √
1 x 2pi PAR sensor
(0046-3097-4.3) √ √ 1 x FastOcean (2220-141-PL) √ √ 2 x USB cables (2292-007-PL) √ √ 2 x 24 V power units (2292-008-PL) √ √ 1 x pumped dark chamber (2220-113-PL) √ √ 1 x profiling frame (2220-111-PL) √ √ 1 x profiling frame interconnect cable (2220-167-PL) √ √ 1 x 24 V dual interface unit (2292-003-PL) √ - 1 x programming / deck cable (2220-170-PL) √ - 1 x programmable 24 V battery pack with 3 x remotes (2220-112-PL) - √ 1 x charger for 24 V battery pack (2220-215-PL) - √ 1 x transit case (2220-204-PL) √ √ System Specifications Mass (weight in air, water) 16 kg (157 N, 63 N) 20.3 kg (200 N, 98 N) Dimensions W 316 x D 292 x H 685 mm W 316 x D 292 x H 685 mm Time between charging for battery system - 7 hrs Power consumption for real time system 16 Watts -
- System Programming
System Programming (FastPro8) The FastOcean-based APD profiling system can be programmed for autonomous operation, if the system Is to be run using the programmable battery pack, or adjusted in real time through FastPro8.
- A complex series of protocols can be set to run in a
continuous, user-defined loop sequence…
- Continuous sequence acquisition at 10 Hz (typical)
- LED intensities are set in meaningful units (photons
m-2 s-1 x 1022)
- Settings changes are applied to ambient and dark sensors simultaneously
- Once set, a protocol series can be saved and retrieved
Data Handling & Analysis FastPro8 incorporates an extensive set of tools for real time data analysis and presentation. Post-processing of downloaded APD data from autonomous profiling systems is also highly automated.
- FRR data can be displayed on scales from the individual acquisition (as shown left) to a time sequence of selected parameters
- Ambient and dark APD data can be shown simultaneously
- Post-processing tools include the facility to subtract blanks for each sensor…
A number of options for data export in a number of different formats are also provided, to allow for secondary analysis. These formats are directly compatible with Excel and MATLAB, for example, and can also be saved as tab-delimited text data.
- A complex series of protocols can be set to run in a
Fast Repetition Rate Fluorometry in the Press:
Oxborough, K. Moore, C.M., Suggett, D.J., Lawson, T., Chan, H.G. and Geider, R.G. Direct estimation of functional PSII reaction centre concentration and PSII electron flux on a volume basis: a new approach to the analysis of Fast Repetition Rate fluorometry (FRRf) data. Liminology & Oceanography: Methods – 2012, 10:142-154. Link to article. Download pdf.